Overexpression of a pine Dof transcription factor in hybrid poplars: A comparative study in trees growing under controlled and natural conditions.
Identifieur interne : 001274 ( Main/Exploration ); précédent : 001273; suivant : 001275Overexpression of a pine Dof transcription factor in hybrid poplars: A comparative study in trees growing under controlled and natural conditions.
Auteurs : Marina Rueda-L Pez [Espagne] ; María Belén Pascual [Espagne] ; Mercedes Pallero [Espagne] ; Luisa María Henao [Espagne] ; Berta Lasa [Espagne] ; Ivan Jauregui [Espagne] ; Pedro M. Aparicio-Tejo [Espagne] ; Francisco M. Cánovas [Espagne] ; Concepci N Ávila [Espagne]Source :
- PloS one [ 1932-6203 ] ; 2017.
Descripteurs français
- KwdFr :
- Arbres (croissance et développement), Arbres (génétique), Arbres (métabolisme), Azote (métabolisme), Biomasse (MeSH), Carbone (métabolisme), Cellulose (métabolisme), Culture hydroponique (MeSH), Dosage génique (MeSH), Facteurs de transcription (génétique), Facteurs de transcription (métabolisme), Gènes de plante (MeSH), Hybridation génétique (MeSH), Lignine (métabolisme), Métabolisme glucidique (génétique), Pinus (génétique), Pinus (métabolisme), Populus (croissance et développement), Populus (génétique), Populus (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes au cours du développement (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Régulation positive (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- croissance et développement : Arbres, Populus.
- génétique : Arbres, Facteurs de transcription, Métabolisme glucidique, Pinus, Populus, Protéines végétales.
- métabolisme : Arbres, Azote, Carbone, Cellulose, Facteurs de transcription, Lignine, Pinus, Populus, Protéines végétales.
- Biomasse, Culture hydroponique, Dosage génique, Gènes de plante, Hybridation génétique, Régulation de l'expression des gènes au cours du développement, Régulation de l'expression des gènes végétaux, Régulation positive, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Biomass (MeSH), Carbohydrate Metabolism (genetics), Carbon (metabolism), Cellulose (metabolism), Gene Dosage (MeSH), Gene Expression Regulation, Developmental (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Plant (MeSH), Hybridization, Genetic (MeSH), Hydroponics (MeSH), Lignin (metabolism), Nitrogen (metabolism), Pinus (genetics), Pinus (metabolism), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (MeSH), Populus (genetics), Populus (growth & development), Populus (metabolism), Transcription Factors (genetics), Transcription Factors (metabolism), Trees (genetics), Trees (growth & development), Trees (metabolism), Up-Regulation (MeSH).
- MESH :
- chemical , genetics : Plant Proteins, Transcription Factors.
- chemical , metabolism : Carbon, Cellulose, Lignin, Nitrogen, Plant Proteins, Transcription Factors.
- genetics : Carbohydrate Metabolism, Pinus, Populus, Trees.
- growth & development : Populus, Trees.
- metabolism : Pinus, Populus, Trees.
- Biomass, Gene Dosage, Gene Expression Regulation, Developmental, Gene Expression Regulation, Plant, Genes, Plant, Hybridization, Genetic, Hydroponics, Plants, Genetically Modified, Up-Regulation.
Abstract
In this work, the role of the pine transcriptional regulator Dof 5 in carbon and nitrogen metabolism has been examined in poplar trees. The overexpression of the gene and potential effects on growth and biomass production were compared between trees growing in a growth chamber under controlled conditions and trees growing in a field trial during two growth seasons. Ten-week-old transgenic poplars exhibited higher growth than untransformed controls and exhibited enhanced capacity for inorganic nitrogen uptake in the form of nitrate. Furthermore, the transgenic trees accumulated significantly more carbohydrates such as glucose, fructose, sucrose and starch. Lignin content increased in the basal part of the stem likely due to the thicker stem of the transformed plants. The enhanced levels of lignin were correlated with higher expression of the PAL1 and GS1.3 genes, which encode key enzymes involved in the phenylalanine deamination required for lignin biosynthesis. However, the results in the field trial experiment diverged from those observed in the chamber system. The lines overexpressing PpDof5 showed attenuated growth during the two growing seasons and no modification of carbon or nitrogen metabolism. These results were not associated with a decrease in the expression of the transgene, but they can be ascribed to the nitrogen available in the field soil compared to that available for growth under controlled conditions. This work highlights the paramount importance of testing transgenic lines in field trials.
DOI: 10.1371/journal.pone.0174748
PubMed: 28376100
PubMed Central: PMC5380328
Affiliations:
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study in trees growing under controlled and natural conditions.</title><author><name sortKey="Rueda L Pez, Marina" sort="Rueda L Pez, Marina" uniqKey="Rueda L Pez M" first="Marina" last="Rueda-L Pez">Marina Rueda-L Pez</name><affiliation wicri:level="4"><nlm:affiliation>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Málaga, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Málaga</wicri:regionArea><orgName type="university">Université de Malaga</orgName><placeName><settlement type="city">Malaga</settlement><region nuts="2" type="region">Andalousie</region></placeName></affiliation></author><author><name sortKey="Pascual, Maria Belen" sort="Pascual, Maria Belen" uniqKey="Pascual M" first="María Belén" last="Pascual">María Belén Pascual</name><affiliation wicri:level="4"><nlm:affiliation>Departamento de Biología 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Lasa</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Ciencias del Medio Natural, Universidad Pública de Navarra, Pamplona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Ciencias del Medio Natural, Universidad Pública de Navarra, Pamplona</wicri:regionArea><wicri:noRegion>Pamplona</wicri:noRegion></affiliation></author><author><name sortKey="Jauregui, Ivan" sort="Jauregui, Ivan" uniqKey="Jauregui I" first="Ivan" last="Jauregui">Ivan Jauregui</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Ciencias del Medio Natural, Universidad Pública de Navarra, Pamplona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Ciencias del Medio Natural, Universidad Pública de Navarra, Pamplona</wicri:regionArea><wicri:noRegion>Pamplona</wicri:noRegion></affiliation></author><author><name sortKey="Aparicio Tejo, Pedro M" sort="Aparicio Tejo, Pedro M" uniqKey="Aparicio Tejo P" first="Pedro M" last="Aparicio-Tejo">Pedro M. Aparicio-Tejo</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Ciencias del Medio Natural, Universidad Pública de Navarra, Pamplona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Ciencias del Medio Natural, Universidad Pública de Navarra, Pamplona</wicri:regionArea><wicri:noRegion>Pamplona</wicri:noRegion></affiliation></author><author><name sortKey="Canovas, Francisco M" sort="Canovas, Francisco M" uniqKey="Canovas F" first="Francisco M" last="Cánovas">Francisco M. Cánovas</name><affiliation wicri:level="4"><nlm:affiliation>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Málaga, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Málaga</wicri:regionArea><orgName type="university">Université de Malaga</orgName><placeName><settlement type="city">Malaga</settlement><region nuts="2" type="region">Andalousie</region></placeName></affiliation></author><author><name sortKey="Avila, Concepci N" sort="Avila, Concepci N" uniqKey="Avila C" first="Concepci N" last="Ávila">Concepci N Ávila</name><affiliation wicri:level="4"><nlm:affiliation>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Málaga, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Málaga</wicri:regionArea><orgName type="university">Université de Malaga</orgName><placeName><settlement type="city">Malaga</settlement><region nuts="2" type="region">Andalousie</region></placeName></affiliation></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biomass (MeSH)</term><term>Carbohydrate Metabolism (genetics)</term><term>Carbon (metabolism)</term><term>Cellulose (metabolism)</term><term>Gene Dosage (MeSH)</term><term>Gene Expression Regulation, Developmental (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Hybridization, Genetic (MeSH)</term><term>Hydroponics (MeSH)</term><term>Lignin (metabolism)</term><term>Nitrogen (metabolism)</term><term>Pinus (genetics)</term><term>Pinus (metabolism)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Populus (metabolism)</term><term>Transcription Factors (genetics)</term><term>Transcription Factors (metabolism)</term><term>Trees (genetics)</term><term>Trees (growth & development)</term><term>Trees (metabolism)</term><term>Up-Regulation (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arbres (croissance et développement)</term><term>Arbres (génétique)</term><term>Arbres (métabolisme)</term><term>Azote (métabolisme)</term><term>Biomasse (MeSH)</term><term>Carbone (métabolisme)</term><term>Cellulose (métabolisme)</term><term>Culture hydroponique (MeSH)</term><term>Dosage génique (MeSH)</term><term>Facteurs de transcription (génétique)</term><term>Facteurs de transcription (métabolisme)</term><term>Gènes de plante (MeSH)</term><term>Hybridation génétique (MeSH)</term><term>Lignine (métabolisme)</term><term>Métabolisme glucidique (génétique)</term><term>Pinus (génétique)</term><term>Pinus (métabolisme)</term><term>Populus (croissance et développement)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Régulation de l'expression des gènes au cours du développement (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Régulation positive (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carbon</term><term>Cellulose</term><term>Lignin</term><term>Nitrogen</term><term>Plant Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Arbres</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Carbohydrate Metabolism</term><term>Pinus</term><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arbres</term><term>Facteurs de transcription</term><term>Métabolisme glucidique</term><term>Pinus</term><term>Populus</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Pinus</term><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arbres</term><term>Azote</term><term>Carbone</term><term>Cellulose</term><term>Facteurs de transcription</term><term>Lignine</term><term>Pinus</term><term>Populus</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biomass</term><term>Gene Dosage</term><term>Gene Expression Regulation, Developmental</term><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Hybridization, Genetic</term><term>Hydroponics</term><term>Plants, Genetically Modified</term><term>Up-Regulation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biomasse</term><term>Culture hydroponique</term><term>Dosage génique</term><term>Gènes de plante</term><term>Hybridation génétique</term><term>Régulation de l'expression des gènes au cours du développement</term><term>Régulation de l'expression des gènes végétaux</term><term>Régulation positive</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this work, the role of the pine transcriptional regulator Dof 5 in carbon and nitrogen metabolism has been examined in poplar trees. The overexpression of the gene and potential effects on growth and biomass production were compared between trees growing in a growth chamber under controlled conditions and trees growing in a field trial during two growth seasons. Ten-week-old transgenic poplars exhibited higher growth than untransformed controls and exhibited enhanced capacity for inorganic nitrogen uptake in the form of nitrate. Furthermore, the transgenic trees accumulated significantly more carbohydrates such as glucose, fructose, sucrose and starch. Lignin content increased in the basal part of the stem likely due to the thicker stem of the transformed plants. The enhanced levels of lignin were correlated with higher expression of the PAL1 and GS1.3 genes, which encode key enzymes involved in the phenylalanine deamination required for lignin biosynthesis. However, the results in the field trial experiment diverged from those observed in the chamber system. The lines overexpressing PpDof5 showed attenuated growth during the two growing seasons and no modification of carbon or nitrogen metabolism. These results were not associated with a decrease in the expression of the transgene, but they can be ascribed to the nitrogen available in the field soil compared to that available for growth under controlled conditions. This work highlights the paramount importance of testing transgenic lines in field trials.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28376100</PMID><DateCompleted><Year>2017</Year><Month>08</Month><Day>28</Day></DateCompleted><DateRevised><Year>2019</Year><Month>02</Month><Day>08</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>12</Volume><Issue>4</Issue><PubDate><Year>2017</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>Overexpression of a pine Dof transcription factor in hybrid poplars: A comparative study in trees growing under controlled and natural conditions.</ArticleTitle><Pagination><MedlinePgn>e0174748</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0174748</ELocationID><Abstract><AbstractText>In this work, the role of the pine transcriptional regulator Dof 5 in carbon and nitrogen metabolism has been examined in poplar trees. The overexpression of the gene and potential effects on growth and biomass production were compared between trees growing in a growth chamber under controlled conditions and trees growing in a field trial during two growth seasons. Ten-week-old transgenic poplars exhibited higher growth than untransformed controls and exhibited enhanced capacity for inorganic nitrogen uptake in the form of nitrate. Furthermore, the transgenic trees accumulated significantly more carbohydrates such as glucose, fructose, sucrose and starch. Lignin content increased in the basal part of the stem likely due to the thicker stem of the transformed plants. The enhanced levels of lignin were correlated with higher expression of the PAL1 and GS1.3 genes, which encode key enzymes involved in the phenylalanine deamination required for lignin biosynthesis. However, the results in the field trial experiment diverged from those observed in the chamber system. The lines overexpressing PpDof5 showed attenuated growth during the two growing seasons and no modification of carbon or nitrogen metabolism. These results were not associated with a decrease in the expression of the transgene, but they can be ascribed to the nitrogen available in the field soil compared to that available for growth under controlled conditions. This work highlights the paramount importance of testing transgenic lines in field trials.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rueda-López</LastName><ForeName>Marina</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pascual</LastName><ForeName>María Belén</ForeName><Initials>MB</Initials><AffiliationInfo><Affiliation>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pallero</LastName><ForeName>Mercedes</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Henao</LastName><ForeName>Luisa María</ForeName><Initials>LM</Initials><AffiliationInfo><Affiliation>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lasa</LastName><ForeName>Berta</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Departamento de Ciencias del Medio Natural, Universidad Pública de Navarra, Pamplona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jauregui</LastName><ForeName>Ivan</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Departamento de Ciencias del Medio Natural, Universidad Pública de Navarra, Pamplona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aparicio-Tejo</LastName><ForeName>Pedro M</ForeName><Initials>PM</Initials><AffiliationInfo><Affiliation>Departamento de Ciencias del Medio Natural, Universidad Pública de Navarra, Pamplona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cánovas</LastName><ForeName>Francisco M</ForeName><Initials>FM</Initials><AffiliationInfo><Affiliation>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ávila</LastName><ForeName>Concepción</ForeName><Initials>C</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-8817-7529</Identifier><AffiliationInfo><Affiliation>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>04</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical><Chemical><RegistryNumber>7440-44-0</RegistryNumber><NameOfSubstance UI="D002244">Carbon</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>N762921K75</RegistryNumber><NameOfSubstance UI="D009584">Nitrogen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="N">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050260" MajorTopicYN="N">Carbohydrate Metabolism</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002244" MajorTopicYN="N">Carbon</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018628" MajorTopicYN="N">Gene Dosage</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018507" MajorTopicYN="N">Gene Expression Regulation, Developmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006824" MajorTopicYN="N">Hybridization, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018527" MajorTopicYN="N">Hydroponics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009584" MajorTopicYN="N">Nitrogen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D028223" MajorTopicYN="N">Pinus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015854" MajorTopicYN="N">Up-Regulation</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>01</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>03</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>4</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>4</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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IdType="pubmed">24094056</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li></country><region><li>Andalousie</li></region><settlement><li>Malaga</li></settlement><orgName><li>Université de Malaga</li></orgName></list><tree><country name="Espagne"><region name="Andalousie"><name sortKey="Rueda L Pez, Marina" sort="Rueda L Pez, Marina" uniqKey="Rueda L Pez M" first="Marina" last="Rueda-L Pez">Marina Rueda-L Pez</name></region><name sortKey="Aparicio Tejo, Pedro M" sort="Aparicio Tejo, Pedro M" uniqKey="Aparicio Tejo P" first="Pedro M" last="Aparicio-Tejo">Pedro M. Aparicio-Tejo</name><name sortKey="Avila, Concepci N" sort="Avila, Concepci N" uniqKey="Avila C" first="Concepci N" last="Ávila">Concepci N Ávila</name><name sortKey="Canovas, Francisco M" sort="Canovas, Francisco M" uniqKey="Canovas F" first="Francisco M" last="Cánovas">Francisco M. Cánovas</name><name sortKey="Henao, Luisa Maria" sort="Henao, Luisa Maria" uniqKey="Henao L" first="Luisa María" last="Henao">Luisa María Henao</name><name sortKey="Jauregui, Ivan" sort="Jauregui, Ivan" uniqKey="Jauregui I" first="Ivan" last="Jauregui">Ivan Jauregui</name><name sortKey="Lasa, Berta" sort="Lasa, Berta" uniqKey="Lasa B" first="Berta" last="Lasa">Berta Lasa</name><name sortKey="Pallero, Mercedes" sort="Pallero, Mercedes" uniqKey="Pallero M" first="Mercedes" last="Pallero">Mercedes Pallero</name><name sortKey="Pascual, Maria Belen" sort="Pascual, Maria Belen" uniqKey="Pascual M" first="María Belén" last="Pascual">María Belén Pascual</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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